Pyrophosphate: Fructose-6-Phosphate-1-Phosphoric Acid Transferase(PFP) Activity Assay Kit, 50T/48SSample

Determination of Significance： Pyrophosphate: Fructose-6-phosphate-1-phosphotransferase (PFP, EC2.7.1.90) is a cytosolic enzyme that is widely present in plant tissues and catalyzes the phosphorylation of fructose-6-phosphate like phosphofructokinase. As a result, the single PEP catalytic reaction is a reversible reaction, and pyrophosphate is used instead of ATP, which plays an important role in carbon metabolism of photosynthesis. Measurement Principle： PFP catalyzes the conversion of fructose 6-phosphate to fructose 1,6-diphosphate, which is converted to dihydroxyacetone phosphate by the action of aldolase and triose phosphate isomerase, and then catalyzed by α-phosphate glycerol dehydrogenase and NADH to from Glycerol 3-diphosphate and NAD. The change in absorbance at 340 nm reflects the level of PFP activity. Self Provided： Low temperture centrifuge, spectrophotometer/microplate reader, water bath/constant temperature incubator, mortar/homogenizer, micro quartz cuvette/96 well plate, transferpettor, ice and distilled water, EP tube. Note： Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

• Size: 50T/48SSample
• Storage: Store at -20℃, 6 months
• Brand: Saint Michael
• Catalogue No.: BC3400-50T/48SSample

For laboratory research use only. Not for human or animal consumption, diagnostic or therapeutic use.