Transhydrogenase-2 Activity Assay Kit, 50T/24SSample

Determination of Significance： S-NR catalyzes the reduction of nitrate to nitrite in soil, which is the key enzyme of nitrate reduction in soil. Study on the activity of S-NR is of great significance for rational fertilization and reduction of nitrogen loss. Measurement Principle： S-NR catalyzes the reduction of nitrate to nitrite, NO 3- +NADH+H+→NO 2- +NAD++H2O; the generated nitrite can quantitatively generate red azo compounds with p-aminobenzenesulfonic acid and α-naphthylamine under acidic conditions; The unreacted NADH will inhibit the subsequent color reaction, and then carry out the subsequent reaction with PMS; the generated red azo compounds are 520 nm has a maximum absorption peak, which can be determined by spectrophotometry. Self Provided： Spectrophotometer/microplate reader, water bath, centrifuge, adjustable pipette, 30 mesh sieve (or smaller), micro glass cuvette/96 well flat-bottom plate, ice and distilled water. Note： Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

• Size: 50T/24SSample
• Storage: Store at 2-8℃, 1 year
• Brand: Saint Michael
• Catalogue No.: BC3260-50T/24SSample

For laboratory research use only. Not for human or animal consumption, diagnostic or therapeutic use.