[Tyr0]Bradykinin, 5mg

Substrate V, Mca-RPPGFSAFK(Dnp), is a very sensitive internally quenched fluorescent substrate for endothelin-converting enzyme-1 (ECE-1) a membrane-bound zinc metallopeptidase that is related to neprilysin in amino acid sequence. The kcat/Km value for the hydrolysis by ECE-1 was 1.9 x 10 7 M -1 s -1 and 1.7 x 10 6 M -1 s -1 for the hydrolysis by neprilysin. This FRET substrate was hydrolyzed rapidly by stromelysin 1 (MMP-3) with kcat/Km= 218000 M -1 s -1 and very slowly by gelatinase B (MMP-9) (kcat/Km= 10100 M -1 s -1 ). There was no hydrolysis by MMP-1 and MMP-2. Mca-RPPGFSAFK(Dnp) has also found use as substrate for insulin-degrading enzyme (IDE) and ACE2.

• Size: 5mg
• Purity: ≥95%
• CAS Number: No.33289-76-8
• Molecular Formula: C59H82N16O13
• Molecular Weight: 1223.4
• Physical State: Lyophilized powder
• Storage: Store at -20℃.(Avoid freeze/thaw cycles)
• Brand: Saint Michael
• Catalogue No.: CLP0990-5mg

For laboratory research use only. Not for human or animal consumption, diagnostic or therapeutic use.